The Investigation of Hydrophobic Lipoproteins Assembly Mechanism
Chia-Yu Chang1*, Hsueh-Liang Chu1, Chun-Ching Lin1, Po-Yen Lin2, Chia-Ching Chang1,3
1Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan
2Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan
3Institute of Physics, Academia Sinica, Taipei, Taiwan
* Presenter:Chia-Yu Chang,
Extremely hydrophobic lipoproteins assembling is one of the important research topics in biological physics. In previous study, we have been demonstrated that the low density lipoprotein (LDL) can be isolated and refolded/constituted by bile salt added refolding process. In this study the refolding process has been demonstrated again and the reconstituted lipoparticle (rABL) is analyzed by GSD (ground state depletion) super resolution fluorescence microscopy. A low resolution rABL structure can be observed at single molecule level. Furthermore, the interaction between rABL and ApoC3 can be observed in this study. The pre-VLDL formation mechanism will be revealed in this study. Furthermore, rABL can be used as anti-cancer drug delivery vehicle. By encapsulating the radicicol with rABL (Rad@rABL), the efficacy enhances 100 times. These results indicate that rABL can be used to study pre-VLD formation but also play the role of drug delivery system, too.

We thank that Ministry of Science and Technology of Taiwan supported this work under grants MOST 103-2122-M-009-011-MY3, and MOST 105-2811-M-009-088.

Keywords: low density lipoprotein (LDL), reconstituted lipoparticle (rABL), GSD super resolution fluorescence microscopy